Fottea, 2014 (vol. 14), issue 1

Fottea 14(1): 63-76, 2014 | DOI: 10.5507/fot.2014.005

Strain survey on three continents confirms the polyphyly of the genus Pediastrum (Hydrodictyaceae, Chlorophyceae).

Mrutyunjay Jena1,2,3, Christina Bock1,4, Chhandashree Behera1,2, Siba P. Adhikary5, Lothar Krienitz*,1
1 Leibniz-Institute of Freshwater Ecology and Inland Fisheries, Alte Fischerhütte2, D-16775 Stechlin- Neuglobsow, Germany
2 Department of Botany, Utkal University, Bhubaneswar-756014, Odisha, India
3 Department of Botany, Berhampur University, Berhampur-760007, Odisha, India
4 University of Essen, Faculty of Biology, D-45141 Essen, Germany
5 Department of Biotechnology, Visva-Bharati Santiniketan-731235, West Bengal, India

Approximately 100 new strains of the Pediastrum-phenotype were isolated from inland waters of India, eastern and southwestern Africa, and Germany and were studied by light and scanning electron microscopy. The 18S rRNA and ITS genes of 28 strains were sequenced and compared with 25 sequences of
Hydrodictyaceae from the GenBank database. Phylogenetic analyses confirmed the polyphyly of Pediastrum. The new species Sorastrum pediastriforme was described. This species resembled morphologically Pediastrum, however, the phylogenetic analyses revealed its affiliation within the genus Sorastrum. Furthermore, the following new combinations: Monactinus sturmii, Pseudopediastrum alternans, Pp. brevicorne, Pp. integrum, and Pp. pearsonii were established.

Keywords: CBC, ITS, Lacunastrum, Monactinus, morphology, Pediastrum, phylogeny, Pseudopediastrum, Sorastrum, SSU, Stauridium

Received: September 13, 2013; Accepted: December 18, 2013; Published: March 1, 2014

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