Fottea 2017, 17(1):20-33 | DOI: 10.5507/fot.2016.011

Taxonomic status of Chara tenuspina A. Br. (Streptophyta: Charales) based on LM morphology, matK, atpB and rbcL of cpDNA sequences

Jacek Urbaniak*1, Micha³ Combik2
1 Department of Botany and Plant Ecology, Wroc³aw University of Environmental and Life Sciences, pl. Grunwaldzki 24a , 50-363 Wroc³aw, Poland; *Corresponding author e-mail: jacek.urbaniak@uap.wroc.pl
2 W. Szafer Institute of Botany, Polish Academy of Science, ul. Lubicz 46, 31-512, Kraków, Poland

Chara tenuispina A. Br. is an extremely rare species of the genus Chara L., which inhabits freshwater and shaded, shallow water environments on calcareous peat bogs, as compared to C. globularis Thuill., which is a widespread species. To re-examine the taxonomic system proposed by Wood & Imahori (1965), who treated C. tenuispina as a variety of C. globularis, we studied the morphology of both species, as well as their phylogenetic relationships, based on three cpDNA gene sequences (atpB, matK, rbcL). In general, the species do not differ significantly in their general appearance, but certain details, such as prolonged stipulodes in upper rows below the branchlet, or the extremely long spine cells in C. tenuispina, may be noted as distinguishing characteristics. In addition, the results of sequence analysis demonstrated that these species are phylogenetically separated, forming distinct clades. This supports the taxonomic interpretation that C. tenuispina is a distinct species rather than variety of C. globularis.

Keywords: Chara tenuispina, Chara globularis, charophytes, dimensions, Europe, morphology, molecular, phylogeny, taxonomy

Received: January 4, 2016; Accepted: April 6, 2016; Prepublished online: January 4, 2017; Published: April 1, 2017  Show citation

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Urbaniak, J., & Combik, M. (2017). Taxonomic status of Chara tenuspina A. Br. (Streptophyta: Charales) based on LM morphology, matK, atpB and rbcL of cpDNA sequences. Fottea, 17(1), 20-33. doi: 10.5507/fot.2016.011
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