RT Journal Article
SR Electronic
A1 Sviežená, Barbara
A1 Gálová, Eliška
A1 Kusenda, Branislav
A1 Slaninová, Miroslava
A1 Vlček, Daniel
A1 Dušinská, Mária
T1 The Comet assay and the troubles with its application in the green alga Chlamydomonas reinhardtii
JF Czech Phycology
YR 2004
VO 4
IS 1
SP 163
OP 174
UL https://fottea.czechphycology.cz/artkey/fot-200401-0015.php
AB The single cell gel electrophoresis or Comet assay is a sensitive, reliable, rapid and economic method for DNA double- and single-strand breaks, alkali-labile sites and delayed repair site detection, in eukaryotic individual cells. Given its overall characteristics, this method has been widely used over the past few years in several different areas. In this paper we review briefly the basic data about the principles, methodology and applications of this assay and we discuss also the possible explanation for not achieving comets to date in singlecell green alga Chlamydomonas reinhardtii.