RT Journal Article
SR Electronic
A1 Škaloud, Pavel
A1 Radochová, Barbora
T1 Confocal microscopy of the green-algal chloroplast
JF Czech Phycology
YR 2004
VO 4
IS 1
SP 183
OP 190
UL https://fottea.czechphycology.cz/artkey/fot-200401-0017.php
AB The confocal microscopy proves useful in the investigations of chloroplast morphology and ontogeny of several coccal green algae. The three-dimensional images allow the reconstruction of the chloroplast morphology and the exact determination of the chloroplast number in the cell. This character is essential for species determination in numerous genera of coccal green algae. The simple method of pyrenoid visualisation by confocal images is demonstrated. Within the pyrenoid we can recognise the structure and shape of the invaginated thylakoids. In comparison with the methodically difficult transmission electron microscopy, the confocal microscopy provides a simple and appropriate method for the morphological and taxonomic studies of various algal taxa.