RT Journal Article
SR Electronic
A1 Hrouzek, Pavel
A1 Kopecký, Jiří
A1 Salát, Jíří
A1 Maršálek, Blahoslav
A1 Lukešová, Alena
T1 Cytotoxic effect of soil cyanobacterial extracts to mammal cell lines YAC-1 and WEHI
JF Czech Phycology
YR 2005
VO 5
IS 1
SP 79
OP 90
UL https://fottea.czechphycology.cz/artkey/fot-200501-0006.php
AB The cytotoxic effects of ten methanol extracts obtained from soil filamentous cyanobacteria (Anabaena, Calothrix, Nodularia, Cylindrospermum, Tolypothrix and Trichormus) were studied. Two different mammal cell lines (YAC-1, WEHI) were selected for cytotoxicity testing using the MTT test and flow-cytometry (FC). For comparison, the brine shrimp assay (using Artemia as a testing organism) was performed. The composition of extracts was studied using HPLC-MS. Both MTT and FC found the cytotoxic effect in 6 of 10 tested cyanobacterial extracts, but these results did not correlate with those obtained by brine shrimp assay. In Anabaena torulosa and Cylindrospermum sp. the most severe damage was recorded by both methods. In cells treated by the extract of Anabaena torulosa, 100% inhibition was found already after 60-minute exposure. Induction of necrosis was revealed by FC, since all tested cells were marked by propidium iodide indicating disruption of the cellular membrane. Surprisingly, no effects associated with this extract were found for Artemia. In Cylindrospermum sp. both necrotic (80%) and apoptotic (20%) induction was found by FC. The presence of microcystins in these extracts was not proved by HPLC-MS, but several peaks with unknown molecular masses were observed. Thus, the production of new cytotoxins with cytoxicity comparable to microcystin can be expected.