RT Journal Article
SR Electronic
A1 Tawong, Wittaya
A1 Pongcharoen, Pongsanat
A1 Nishimura, Tomohiro
T1 Geosmin-producing Scytonema foetidum sp. nov. (Scytonemataceae, Cyanobacteria): morphology and molecular phylogeny
JF Fottea
YR 2022
VO 22
IS 1
SP 78
OP 92
DO 10.5507/fot.2021.017
UL https://fottea.czechphycology.cz/artkey/fot-202201-0005.php
AB A novel Scytonema species, Scytonema foetidum sp. nov. is described by combining morphological and genetic evidences. Two clonal strains of the new species were isolated from a wet soil sample taken from the Khek River in the lower northern part of Thailand. This species is morphologically similar to S. hofmanii, but can be differentiated by a small filament width, conically rounded apical cell, and sheath present throughout the whole trichome. The 16S ribosomal RNA (rRNA), rbcLX, and nifH phylogenetic trees indicated that S. foetidum clearly formed a unique cluster within the Scytonema sensu stricto clade and shared low 16S rRNA sequence similarity (<98.4%) with the phylogenetically related taxa, supporting separation at species level. The 16S-23S rRNA internal transcribed spacer (ITS) sequences of S. foetidum also possessed unique secondary structures in D1-D1', Box B, and V3 helices, differentiating this species from other known Scytonema species. Furthermore, S. foetidum could produce the odoriferous compound, geosmin.