Czech Phycology 2: 139-142, 2002

Statistical evaluation of algal toxicity bioassays [in Czech]

Statistické vyhodnocování řasových biotestů

Vladimír Kočí, Martin Mlejnek, Jiří Burkhard
Ústav chemie ochrany prostředí, VŠCHT, Technická 5, CZ-160 00 Praha 6

The article deals with the problems of statistical presentation of algal toxicity tests. We performed 12 and 10 parallel cultivations of algal suspensions in standard condition for toxicity assays. We suggest that the deviation up to 15%. determined by Probit analysis is not fully adequate for determination of EC50 for algal bioassays. Log-Lin linear regression fits better for experimental data. Only concentrations with inhibition effects from 20% to approximately 80% are recommended for calculation. For NOEC we recommend to choose the highest tested concentration with inhibition effect under 20%. Consequently, the next higher concentration with inhibition effect > 20% is recommended for LOEC determination.

Published: March 1, 2002  Show citation

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Kočí, V., Mlejnek, M., & Burkhard, J. (2002). Statistical evaluation of algal toxicity bioassays [in Czech]. Czech Phycology, 2(1), 139-142
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    References

    1. ČSN EN 28692 757740. Jakost vod. Zkouška inhibice růstu sladkovodních řas Scenedesmus subspicatus a Selenastrum capricornutum (ISO 8692: 1989).
    2. FINNEY, D.J (1952). Probit Analysis: A Statistical Treatment of the Sigmoid Response Curve (2nd edition). - Cambridge University Press, London.
    3. GOTTWALDOVÁ, V., SVOBODOVÁ, Z. (2001). Konferenci Toxicita a biodegradabilita odpadů a látek významných ve vodním prostředí. Ústní sdělení.
    4. KOČÍ, V.: Statistical questionmarks of the algal bioassays. - Czech Phycology, 1: 121-127, 2001.
    5. KOČÍ, V.(2000) Prezentace výsledků ekotoxikologických dat. ERA 2000, 12.-15.3.2000. TOCOEN, RECETOX. Brno, str. 165-168.
    6. LICHFIELD, J.T., WILCOXON, F.W. (1949) A simplified method of evaluating dose-effect experiments. - J. Pharm. Exp. Therap. 95, 99.

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