Czech Phycology 5: 79-90, 2005

Cytotoxic effect of soil cyanobacterial extracts to mammal cell lines YAC-1 and WEHI

Cytotoxický efekt extraktů půdních sinic k liniím savčích buněk YAC-1 a WEHI

Pavel Hrouzek1,2, Jiří Kopecký2, Jíří Salát3, Blahoslav Maršálek4, Alena Lukešová5
1 University of South Bohemia, Faculty of Biological Sciences, Department of Botany, Branišovská 31, CZ-370 05 České Budějovice
2 Institute of Microbiology, Academy of Sciences of the Czech Republic, Department of Autotrophic Microorganisms Opatovický mlýn, CZ-379 81 Třeboň
3 Institute of Parasitology, Academy of Sciences of the Czech Republic, Branišovská 31, CZ -370 05 České Budějovice
4 Institute of Botany, Academy of Sciences of the Czech Republic, Department of Experimental Phycology and Ecotoxicology, Květná 8, CZ-603 65 Brno
5 Institute of Soil Biology, Academy of Sciences of the Czech Republic, Na Sádkách 7, CZ-370 05 České Budějovice

The cytotoxic effects of ten methanol extracts obtained from soil filamentous cyanobacteria (Anabaena, Calothrix, Nodularia, Cylindrospermum, Tolypothrix and Trichormus) were studied. Two different mammal cell lines (YAC-1, WEHI) were selected for cytotoxicity testing using the MTT test and flow-cytometry (FC). For comparison, the brine shrimp assay (using Artemia as a testing organism) was performed. The composition of extracts was studied using HPLC-MS. Both MTT and FC found the cytotoxic effect in 6 of 10 tested cyanobacterial extracts, but these results did not correlate with those obtained by brine shrimp assay. In Anabaena torulosa and Cylindrospermum sp. the most severe damage was recorded by both methods. In cells treated by the extract of Anabaena torulosa, 100% inhibition was found already after 60-minute exposure. Induction of necrosis was revealed by FC, since all tested cells were marked by propidium iodide indicating disruption of the cellular membrane. Surprisingly, no effects associated with this extract were found for Artemia. In Cylindrospermum sp. both necrotic (80%) and apoptotic (20%) induction was found by FC. The presence of microcystins in these extracts was not proved by HPLC-MS, but several peaks with unknown molecular masses were observed. Thus, the production of new cytotoxins with cytoxicity comparable to microcystin can be expected.

Published: March 1, 2005  Show citation

ACS AIP APA ASA Harvard Chicago Chicago Notes IEEE ISO690 MLA NLM Turabian Vancouver
Hrouzek, P., Kopecký, J., Salát, J., Maršálek, B., & Lukešová, A. (2005). Cytotoxic effect of soil cyanobacterial extracts to mammal cell lines YAC-1 and WEHI. Czech Phycology, 5(1), 79-90
Share...
Download citation
  • BibTeX (.bib)
  • Bookends (.ris)
  • EasyBib (.ris)
  • EndNote (.enw)
  • EndNote 8 (.xml)
  • ISI WoS (.isi)
  • Medlars (.medlars)
  • Mendeley (.ris)
  • MODS (.xml)
  • Papers (.ris)
  • RefWorks (.txt)
  • RefManager (.ris)
  • RIS (.ris)
  • MS Word (.xml)
  • Zotero (.ris)
    • Open full article

    References

    1. BANKER, R. & CARMELI, S. (1999): Inhibitors of serine from a water bloom of the cyanobacterium Microcystis sp. - Tetrahedr. 55: 10835-10844. Go to original source...
    2. BISCHOFF, H. W. & BOLD, H. C. (1963): Phycological Studies. IV. Some soil algae from Enchanted Rock and related algal species. - Univ. Texas Publ., 6318: 1-95.
    3. BOTHA, N., GEHRINGER, M. M.; DOWNING, T. G.; VENTER, M. & SHEPHARD; E. G. (2004): The role of microcystin-LR in the induction of apoptosis and oxidative stress in a CaCo2 cells. - Toxicon 43: 85-92. Go to original source...
    4. CARMELI, S., MOORE, R. E. & PATTERSON, G. M. L. (1991): Mirabimides A-D, new Nacylpyrroines from the blue-green alga Scytonema mirabile. - Tetrahedr. 47: 2087-2096. Go to original source...
    5. CARMELI, S., PAIK, S., MOORE, R. E., PATTERSON, G. M. L. & YOSIDA, W. Y. (1993): Revised structures and biosyntetic studies of tanazoles A and B. - Tetrahedr. Lett. 34(42): 66816684. Go to original source...
    6. CARMICHAEL, W. W. (1986): Algal Toxins. - Adv. Bot. Res. 12: 47-101. Go to original source...
    7. CARMICHAEL, W. W. (1990): Natural toxins from cyanobacteria. - Mar. Tox. 418: 87-106. Go to original source...
    8. CARMICHAEL, W. W. (1992): Cyanobacterial secondary metabolites - a review. - J. App. Bacteriol. 72: 445-459. Go to original source...
    9. CHORUS, I. (2000): Cyanotoxins - occurence, causes, consequences, 1-330. - Springer; Berlin. Go to original source...
    10. DING, W. X., SHEN, H. M., ZHU, H. G. & ONG, C. N. (1998): Studies on oxidative damage induced by cyanobacterial extract in primary cultured rat hepatocytes. - Environ. Res. 78: 12-18. Go to original source...
    11. DING, W. X., SHEN, H. M. & ONG, C. N. (2000): The critical role of ROS and mitochondrial membrane permeability in microcystin-LR induced rapid apoptosis in primary rat hepatocytes. - Hepatol. 32: 547-555. Go to original source...
    12. DING, W. X. & ONG, CH. N. (2003): Role of oxidative stress and mitochondrial changes in cyanobacterial-induced apoptosis and hepatotoxicity. - FEMS Microbiol. Lett. 220: 1-7. Go to original source...
    13. FLANDMARK, K. E., BRUSTUGUN, O. T., HOVLAND, R., BOE, R., GJERTSEN, B. T., ZHIVOTOVSKY, B. & DOSKELAND, S. O. (1999): Ultrarapid capsase-3 dependent apoptosis induction by serine/threonine phosphatase inhibitors. - Cell Death Differ. 6: 1099-1108. Go to original source...
    14. GOLAKOTI, T., YOSHIDA, Y. W., CHAGANTY, S. & MOORE, R. E. (2000): Isolation and structures of nostopeptilides A1,A2 and A3 from cyanobacterium Nostoc sp. GSV 224. Tetrahedr. 56: 9093-9102. Go to original source...
    15. ISHIDA, K., MATSUDA, H., MURAKAMI, M. & YAMAGUCHI, K. (1996): Kawaguchipeptin A, a novel cyclic undecapeptide from cyanobacterium Microcystis aeruginosa (NIES-88). Tetrahedr. 52: 9025-9030. Go to original source...
    16. ISHIDA, K., MATSUDA, H., MURAKAMI, M. & YAMAGUCHI, K. (1997): Microginins 299-A and B, leucine amidopeptidase inhibitors from the cyanobacterium Microcystis aeruginosa (NIES-299). - Tetrahedr. 53(30): 10281-10288. Go to original source...
    17. ISHIDA, K., MATSUDA, H. & MURAKAMI, M. (1997): Four new microginins 299-A, linear peptides from the cyanobacterium Microcystis aeruginosa. - Tetrahedr. 54: 1347513484. Go to original source...
    18. ISHIDA, K., MATSUDA, H., OKINO, T. & MURAKAMI, M. (1999): Aeruginosins, protease inhibitors from the cyanobacterium Mycrocystis aeruginosa. - Tetrahedr. 55: 1097110988. Go to original source...
    19. ISHIDA, K., KATO, T., MURAKAMI, M., WATANABE, M. & WATANABE, M. F. (2000): Microginins, Zinc metaloproteases inhibitors from the cyanobacterium Microcystis aeruginosa. Tetrahedr. - 56: 8643-8656. Go to original source...
    20. JUNG, J. H., MOORE, R. E. & PATTERSON, G. M. L. (1991): Scytophycins from a blue green alga belonging to the Noscocaceae. - Phytochem. 30(11): 3615-3616. Go to original source...
    21. KLEIN, D., DALOZE, J., BRARKMAN C., HOFFMAN, L. & DEMOULIN, V. (1995): New hapalindoles from the cyanophyte hapalosiphon langii. - J. Natur. Prod. 58: 1781-1785. Go to original source...
    22. LINCOLN, R. D., STRUPINSKI, K. & WALKER, J. M. (1996): The use of Artemia naupli (Brine shrimp larvae) to detect toxic compounds from microalgal cultures. - Int. J. Pharm. 34: 384-389. Go to original source...
    23. LUESCH, H., YOSHIDA, W. Y., MOORE, R. E., PAUL, J. V. & MOOREBERRY, S. (2000): Isolation, structure determination, and biological activity of Lyngbyabellin A from the marine cyanobacterium Lyngbya majuscula. - J. Natur. Prod. 63: 611-615. Go to original source...
    24. LUESCH, H., YOSHIDA, W. Y., MOORE, R. E. & PAUL, J. V. (2002): Structurally diverse alkaloids from Palauan collection of the apratoxin-producing marine cyanobacterium Lyngbya sp. Tetrahedr. 58: 7959-7966. Go to original source...
    25. MARŠÁLEK, B., KERŠNER, V. & MARVAN, P. (ed.) (1996): Vodní květy sinic. 1-140 [Waterblooms of the cyanobacteria] (in Czech). - Nadatio flos aque; Brno.
    26. MATERN, U., OBERER, L., FALCHETTO, R. A., ERHARD, M., KÖNIG, W. A., HERDMAN, M. & WECKESSER, J. (2001): Scyptolin A and B, cyclic depsipeptides from axenic cultures of Scytonema hofmanii PCC 7110. - Phytochem. 58: 1087-1095. Go to original source...
    27. MATERN, U., OBERER, L., ERHARD, M., HERDMAN, M. & WECKESSER, J. (2003): Hofmanolin, a cyanopeptolin from Scytonema hofmanii PCC 7110. - Phytochem. 64: 1061-1067. Go to original source...
    28. MCDERMOTT, C. M., NHO, C. V., HOWARD, W. & HOLTON, B. (1998): The cyanobacterial toxin, microcystin-LR, can induce apoptosis ina variaety o cell types. - Toxicon 36: 1981-1996. Go to original source...
    29. MIAN, P., HEILMANN, J., BURGI, H.R., STICHER, O. (2000): Biological screening of terrestrial and freshwater cyanobacteria for antimicrobial activity, brine shrimp lethality, and cytotoxicity. - Pharm. Biol. 41: 243-247. Go to original source...
    30. MOORE, G. E., GERNER, R., E. & FRANKLIN, H. A. (1967): Culture of normal human leukocytes. - JAMA 199: 519-534. Go to original source...
    31. MOSMANN, T. (1983): Rapid colometric assay for cellular growth and survival: aplication to proliferation ans cytotoxicity assay. - J. Imun. Meth. 65: 55-63. Go to original source...
    32. PATTERSON, G. M. L & CARMELI, S. (1992): Biological effects of tolytoxin (6-hydroxy-7-Omethyl-scytophycin b) a potent bioactive metabolite from cyanobacteria. - Arch. Microbiol. 157: 406-410. Go to original source...
    33. PICCARDI, R., FROSINI, A., TREDICI, M. R. & MARGHERI, M. C. (2000): Bioactivity in freeliving and symbiotic cyanobacteria of the genus Nostoc. - J. Appl. Phycol. 12: 543-547. Go to original source...
    34. RESHEF, V. & CARMELI, S. (2001): Protease inhibitors from a water bloom of the cyanobacterium Microcystis aeruginosa. - Tetrahedr. 57: 2885-2894. Go to original source...
    35. SIVONEN K. (1996): Cyanobacteria toxins and toxin production. - Phycol. 35: 12-24. Go to original source...
    36. SOLIS, P.N., WRIGHT, C. W., ANDERSON, M. M., GUPTA, M. P. & PHILLIPSON, J. D. (1993): A microwell cytotoxicity assay using Artemia-salina (brine shrimp). - Planta Med. 59: 250-252. Go to original source...
    37. TRIMURTULU, G., OHTANI, I., PATTERSON, G. M. L., MOORE, R. E., CORBETT, T. H., VALERIOTE, F. A. & DEMCHIK, L. (1994): Total structures of cryptophycins, potent antitumor depsipeptides from the blue-green alga Nostoc sp. strain GSV 224. - J. A. Chem. Soc. 116: 4729-4737. Go to original source...
    38. YANG, X., SHIMIZU, Y., STEINER, R. J. & CLARDY, J. (1993): Nostoclide I and II, extracellular metabolites from a symbiotic cyanobacterium, Nostoc sp., from the lichen Peltigera canina. - Tetrahedr. Lett. 34: 761-764. Go to original source...
    39. YOSHIZAWA, S., MATSUSHIMA, R., WATANABE, M. F., HARADA, K. I., ICHIHARA, A., CARMICHAEL, W. W. & FUJIKI, H. (1990): Inhibition of protein phosphatases by microcystin and nodularin associated with hepatotoxicity. - J. Canc. Res. Clin. Oncol. 116: 609-614. Go to original source...

    Return to the content