Fottea 2016, 16(1):104-111 | DOI: 10.5507/fot.2016.002

Phylogenetic position of Geitleribactron purpureum (Synechococcales, Cyanobacteria / Cyanophyceae) and its implications for the taxonomy of Chamaesiphonaceae and Leptolyngbyaceae

Jan Mareš1,2,3*, Marco Cantonati4
1 Biology Centre of the CAS, v.v.i., Institute of Hydrobiology, Na Sádkách 7, CZ-37005 České Budějovice, Czech Republic
2 Institute of Botany of the CAS, v.v.i., Centre for Phycology, Dukelská 135, CZ-37982 Třeboň, Czech Republic
3 Faculty of Science, University of South Bohemia, Department of Botany, Branišovská 1760, CZ-37005 České Budějovice, Czech Republic
4 Museo delle Scienze - MUSE, Limnology and Phycology Section, Corso del Lavoro e della Scienza 3, I-38123 Trento, Italy

Over the last decades, the taxonomy of cyanobacteria has been considerably improved and restructured due to the increase in data output from molecular phylogeny. Recently, a new protocol was developed that enables reliable sequencing of 16S rRNA genes in cultivation-resistant cyanobacteria using analysis of single cells, filaments, or colonies. In the current study, we examined a sample of a heteropolar unicellular cyanobacterium, Geitleribactron purpureum, from the holotype material (deep epilithon of Lake Tovel, Western Dolomites, Italy). We isolated and purified single colonies of G. purpureum, and subjected them to direct PCR and 16S rRNA gene sequencing. We obtained a congruent set of sequences that formed a unique, isolated cyanobacterial lineage, showing phylogenetic clustering among simple filamentous genera of the family Leptolyngbyaceae. We provide evidence for deep polyphyly in Chamaesiphonaceae, and suggest that Geitleribactron should be re-classified in the Leptolyngbyaceae.

Keywords: Alps, carbonate lakes, Geitleribactron, heteropolar cyanobacteria, single–colony sequencing, unicellular cyanobacteria

Received: September 21, 2015; Accepted: January 2, 2016; Prepublished online: March 21, 2016; Published: March 30, 2016  Show citation

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Mareš, J., & Cantonati, M. (2016). Phylogenetic position of Geitleribactron purpureum (Synechococcales, Cyanobacteria / Cyanophyceae) and its implications for the taxonomy of Chamaesiphonaceae and Leptolyngbyaceae. Fottea, 16(1), 104-111. doi: 10.5507/fot.2016.002
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    References

    1. Mateo, P.; Fernández-Piñas, F.; Perona, E. & Sabater S. (2014): Special Section: New developments and applications in the use of algae for monitoring rivers. Foreword. - Science of The Total Environment 475: 157. Go to original source...
    2. Whitton, B. (2012): Changing approaches to monitoring during the period of the 'Use of Algae for Monitoring Rivers' symposia. - Hydrobiologia 695: 7-16. Go to original source...
    3. Barinova, S.; Medvedeva, L. & Nevo, E. (2008): Regional influences on algal biodiversity in two polluted rivers of Eurasia (Rudnaya River, Russia, and Qishon River, Israel) by bioindication and canonical correspondence analysis. - Appl. Ecol. Env. Res. 6: 29-59. Go to original source...
    4. Biolo, S. & Rodrigues, L. (2011): Composição de algas perifíticas (exceto Bacillariophyceae) em distintos substratos naturais de um ambiente semilótico, planície de inundação do Alto Rio Paraná, Brasil. - Revista Brasil. Bot. 34: 307-319. Go to original source...
    5. Bohunická, M.; Johansen, J. R. & Fučíková, K. (2011): Tapinothrix clintonii sp. nov. (Pseudanabaenaceae, Cyanobacteria), a new species at the nexus of five genera. - Fottea 11: 127-140. Go to original source...
    6. Cantonati, M.; Guella, G.; Komárek, J. & Spitale, D. (2014b): Depth distribution of epilithic cyanobacteria and pigments in a mountain lake characterized by marked water-level fluctuations. - Freshw. Sci. 33: 537-547. Go to original source...
    7. Cantonati, M.; Guella, G.; Spitale, D.; Angeli, N.; Borsato, A.; Lencioni, V. & Filippi, M.L. (2014c): The contribution of lake benthic algae to the sediment record in a carbonate mountain lake influenced by marked natural water-level fluctuations. - Freshw. Sci. 33: 499-512. Go to original source...
    8. Cantonati, M.; Komárek, J.; Hernández-Mariné, M. & Angeli, N. (2014a): New and poorly known coccoid species (Cyanoprokaryota) from the mid-depth and deep epilithon of a carbonate mountain lake. - Freshw. Sci. 33: 548-556. Go to original source...
    9. Cantonati, M.; Scola, S.; Angeli, N.; Guella, G. & Frassanito, R. (2009): Environmental controls of epilithic diatom depth-distribution in an oligotrophic lake characterised by marked water-level fluctuations. - Eur. J. Phycol. 44: 15-29. Go to original source...
    10. Casamatta, D. A.; Johansen, J. R.; Vis, M. L. & Broadwater, S. T. (2005): Molecular and morphological characterization of ten polar and near-polar strains within the Oscillatoriales (Cyanobacteria). - J. Phycol. 41: 421-438. Go to original source...
    11. Castenholz, R. W. (1992): Species usage, concept, and evolution in the Cyanobacteria (blue-green algae). - J. Phycol. 28:737-745. Go to original source...
    12. Castenholz, R. W. (2001): Phylum BX. Cyanobacteria. - In: Boone, D. R. & Castenholz, R. W. (eds): Bergey's Manual of Systematic Bacteriology. - pp. 473-599, Springer, New York. Go to original source...
    13. Castenholz, R. W. & Norris, T. B. (2005): Revisionary concepts of species in the Cyanobacteria and their applications. - Algological Studies 117: 53-69. Go to original source...
    14. Dvořák, P.; Casamatta, D. A.; Poulíčková, A.; Hašler, P.; Ondřej, V. & Sanges, R. (2014a): Synechococcus: 3 billion years of global dominance. - Mol. Ecol. 23: 5538-5551. Go to original source...
    15. Dvořák, P.; Hindák, F.; Hašler, P.; Hindáková, A. & Poulíčková, A. (2014b): Morphological and molecular studies of Neosynechococcus sphagnicola, gen. et sp. nov. (Cyanobacteria, Chroococcales). - Phytotaxa 170: 24-34. Go to original source...
    16. Dvořák, P.; Poulíčková, A.; Hašler, P.; Belli, M.; Casamatta, D. A. & Papini, A. (2015): Species concepts and speciation factors in cyanobacteria, with connection to the problems of diversity and classification. - Biodivers. Conserv. 24: 739-757. Go to original source...
    17. Gascuel, O. (1997): BIONJ: an improved version of the NJ algorithm based on a simple model of sequence data. - Mol. Biol. Evol. 14: 685-95. Go to original source...
    18. Geitler, L. (1970): Beiträge zur epiphytischen Algenflora des Neusiedler Sees. - Österr. Bot. Ztschr. 118: 17-29. Go to original source...
    19. Geitler., L. (1975). Zur vergleichenden Morphologie der Cyanophycee Clastidium setigerum und verwandten Gattungen. - Plant Syst. Evol. 124: 161-171. Go to original source...
    20. Gold-Morgan, M.; González-Resendiz, L.; León-Tejera, H. & Montejano, G. (2015): Description of coccoid cyanoprokaryote Nisada stipitata morphogen. et sp. nov. from the supralittoral zone in the tropical Mexican Pacific. - Phytotaxa 220: 268-276. Go to original source...
    21. Golubić, S. (1967): Die littorale Algenvegetation des Titisees. - Arch. Hydrobiol./Suppl. 33: 172-205.
    22. Gouy, M.; Guindon, S. & Gascuel, O. (2010): SeaView version 4: a multiplatform graphical user interface for sequence alignment and phylogenetic tree building. - Mol. Biol. Evol. 27: 221-4. Go to original source...
    23. Hall, T. A. 1999. BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95 / 98 / NT. - Nucleic Acids Symp. Ser. 41: 95-8.
    24. Hällfors, G. & Munsterhjelm, R. (1982): Some epiphytic Chamaesiphonales from fresh and brackish water in southern Finland. - Ann. Bot. Fenn. 19: 147-176.
    25. Johansen, J. R. & Casamatta, D. A. (2005): Recognizing cyanobacterial diversity through adoption of a new species paradigm. - Algol. Stud. 117: 71-93. Go to original source...
    26. Karosienė, J. & Kasperovičienė, J. (2008): Epiphyton Chroococcales cyanobacteria species new to algaeflora of Lithuanian freshwaters. - Bot. Lith. 14: 159-169.
    27. Kaštovský, J.; Hauer, T.; Mareš, J.; Krautová, M.; Bešta, T.; Komárek, J.; Desortová, B.; Heteša, J.; Hindáková, A.; Houk, V.; Janeček, E.; Kopp, R.; Marvan, P.; Pumann, P.; Skácelová, O. & Zapomělová., E. (2010): A review of the alien and expansive species of freshwater cyanobacteria and algae in the Czech Republic. - Biol. Invasions 12: 3599-3625. Go to original source...
    28. Katoh, K. & Standley, D. M. (2013): MAFFT multiple sequence alignment software version 7: improvements in performance and usability. - Mol. Biol. Evol. 30: 772-780. Go to original source...
    29. Komárek, J. (1975): Geitleribactron - eine neue, Chamaesiphon-ähnliche Blaualgengattung. - Plant Syst. Evol. 123: 263-281. Go to original source...
    30. Komárek, J. (2014): Phenotypic and ecological diversity of freshwater coccoid cyanobacteria from maritime Antarctica and Islands of NW Weddell Sea. II. - Czech Polar Reports 4: 16-39. Go to original source...
    31. Komárek, J. & Anagnostidis, K. (1998): Cyanoprokaryota I. - In: Ettl, H.; Gärtner, G.; Heynig, H. & Mollenhauer, D. (eds): Süßwasserflora von Mitteleuropa, Band 19/1. - 548 pp., Gustav Fischer Verlag, Stuttgart - Jena.
    32. Komárek, J.; Kaštovský, J.; Mareš, J. & Johansen, J. R. (2014): Taxonomic classification of cyanoprokaryotes (cyanobacterial genera) 2014, using a polyphasic approach. - Preslia 86: 295-335.
    33. Komárek, J.; Kaštovský, J.; Ventura, S.; Turicchia, S. & Šmarda, J. (2009): The cyanobacterial genus Phormidesmis. - Algol. Stud. 129: 41-59. Go to original source...
    34. Loza, V.; Perona, E. & Mateo, P. (2013a): Molecular fingerprinting of cyanobacteria from river biofilms as a water quality monitoring tool. - Appl. Env. Microbiol. 79: 1459-72. Go to original source...
    35. Loza, V.; Berrendero, E.; Perona, E. & Mateo, P. (2013b): Polyphasic characterization of benthic cyanobacterial diversity from biofilms of the Guadarrama River (Spain): morphological, molecular, and ecological approaches. - J. Phycol. 49: 282-297. Go to original source...
    36. Mareš, J.; Lara, Y.; Dadáková, I.; Hauer, T.; Uher, B.; Wilmotte, A. & Kaštovský, J. (2015): Phylogenetic analysis of cultivation-resistant terrestrial cyanobacteria with massive sheaths (Stigonema spp. and Petalonema alatum, Nostocales, Cyanobacteria) using single-cell and filament sequencing of environmental samples. - J. Phycol. 51: 288-297. Go to original source...
    37. McGregor, G. B. (2013): Freshwater Cyanobacteria of North-Eastern Australia: 2. Chroococcales. - Phytotaxa 133: 1-130. Go to original source...
    38. Miller, M. A.; Pfeiffer, W. & Schwartz, T. (2012): The CIPRES science gateway: enabling high-impact science for phylogenetics researchers with limited resources. - In: Proceedings of the 1st Conference of the Extreme Science and Engineering Discovery Environment: Bridging from the eXtreme to the campus and beyond. - pp. 1-8, ACM, Chicago, Illinois. Go to original source...
    39. Niedermayr, R. & Schagerl, M. (2010): Structuring factors of the phytobenthos community along a mountain headwater (Kalkalpen National Park, Austria). - Fundam. Appl. Limnol. 177: 93-104. Go to original source...
    40. Osorio-Santos, K.; Pietrasiak, N.; Bohunická, M.; Miscoe, L. H.; Kováčik, L'.; Martin, M. P. & Johansen, J. R. (2014): Seven new species of Oculatella (Pseudanabaenales, Cyanobacteria). - Eur. J. Phycol. 49: 450-470. Go to original source...
    41. Palinska, K. A. & Surosz, W. (2014): Taxonomy of cyanobacteria: a contribution to consensus approach. - Hydrobiologia 740: 1-11. Go to original source...
    42. Perkerson, R. B.; Johansen, J. R.; Kováčik, Ľ.; Brand, J.; Kaštovský, J. & Casamatta, D. A. (2011): A unique pseudanabaenalean (Cyanobacteria) genus Nodosilinea gen. nov. based on morphological and molecular data. - J. Phycol. 47: 1397-1412. Go to original source...
    43. Rosowski, J. R.; Bielik, I. & Lee, K. W. (1995): Observations on a chamaesiphonaceous alga (Cyanophyceae) with special reference to exocyte formation. - J. Phycol. 31: 435-446. Go to original source...
    44. Rott, E. (2008): Chamaesiphon komárekii species nova, a new benthic freshwater chroococcalean species (Cyanophyta/ Cyanobacteria) from western coniferous forest streams in British Columbia, Canada. - Algol. Stud. 126: 37-46. Go to original source...
    45. Sant´Anna, C. L.; Gama-Jr., W. A.; Azevedo, M. T. P. & Komárek, J. (2011): New morphospecies of Chamaesiphon (Cyanobacteria) from Atlantic rainforest, Brazil. - Fottea 11: 25-30. Go to original source...
    46. Schirrmeister, B. E.; Antonelli, A. & Bagheri, H. C. (2011): The origin of multicellularity in cyanobacteria. - BMC Evol. Biol. 11: 45. Go to original source...
    47. Schirrmeister, B. E.; deVos, J. M.; Antonelli, A. & Bagheri, H. C. (2013): Evolution of multicellularity coincided with increased diversification of cyanobacteria and the Great Oxidation Event. - Proc. Natl. Acad. Sci. USA 110: 1791-1796. Go to original source...
    48. Shih, P. M.; Wu, D.; Latifi, A.; Axen, S. D.; Fewer, D. P.; Talla, E.; Calteau, A.; Cai, F.; Tandeau de Marsac, N.; Rippka, R.; Herdman, M.; Sivonen, K.; Coursin, T.; Laurent, T.; Goodwin, L.; Nolan, M.; Davenport, K. W.; Han, C. S.; Rubin, E. M.; Eisen, J. A.; Woyke, T.; Gugger, M. & Kerfeld, C. A. (2013): Improving the coverage of the cyanobacterial phylum using diversity-driven genome sequencing. - Proc. Natl. Acad. Sci. USA 110:1053-1058. Go to original source...
    49. Stamatakis, A. (2014): RAxML version 8: A tool for phylogenetic analysis and post-analysis of large phylogenies. - Bioinformatics 30: 1312-1313. Go to original source...
    50. Turner, S. (1997): Molecular systematics of oxygenic photosynthetic bacteria. - Plant Syst. Evol. 11: 53-86. Go to original source...
    51. Vaz, M. G. M. V.; Genuário, D. B.; Andreote, A. P. D.; Malone, C. F. S.; Sant´Anna, C. L.; Barbiero, L. & Fiore, M. F. (2015): Pantanalinema gen. nov. and Alkalinema gen. nov.: Novel pseudanabaenacean genera (Cyanobacteria) isolated from saline-alkaline lakes. - Int. J. Syst. Evol. Microbiol. 65: 298-308. Go to original source...
    52. Zammit, G.; Billi, D. & Albertano, P. (2012): The subaerophytic cyanobacterium Oculatella subterranea (Oscillatoriales, Cyanophyceae) gen. et sp. nov.: a cytomorphological and molecular description. - Eur. J. Phycol. 47: 341-354. Go to original source...

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