Fottea 2017, 17(2):209-221 | DOI: 10.5507/fot.2017.005

Genetic diversity in chrysophytes: Comparison of different gene markers

Christina Bock1, Antonis Chatzinotas2,3, Jens Boenigk1,4
1 Department of Biodiversity, University Duisburg-Essen, Universitaetsstrasse 5, D-45117 Essen, Germany
2 Department of Environmental Microbiology, Helmholtz Centre for Environmental Research - UFZ, Permoserstrasse 15, D-04318 Leipzig, Germany
3 German Centre for Integrative Biodiversity Research (iDiv) Halle-Jena-Leipzig, Deutscher Platz 5e, 04103 Leipzig, Germany
4 Centre for Water and Environmental Research (ZWU), University of Duisburg-Essen, Universitaetsstr. 2, 45117 Essen, Germany

Chrysophytes are a diverse group of protists belonging to the Stramenopiles. They encompass a broad range of nutrition strategies (phototrophy, mixotrophy and heterotrophy) and morphological forms (unicellular, colonial, branched, non-scaled to silica-scaled). However, taxonomy, identification and delineation of genera and species are hampered by the polyphyletic origin of several morphotypes and the low resolution of morphological features; moreover, description of these features requires in many cases special equipment like TEM or SEM. To test the congruent phylogeny, genetic resolution and to reveal the genetic diversity of chrysophytes, we evaluated in this study different gene markers, in particular SSU and LSU rRNA genes, ITS, and COI in terms of phylogenetic clustering, separation of phylogenetic lineages and genetic distances within and between clades. Our results showed that the general topology of the SSU rRNA gene phylogeny is similar to that of the LSU rRNA gene phylogeny, with a higher genetic divergence for the latter. The topology of the COI phylogeny differed from the ribosomal data, and showed the highest genetic divergence within groups and between groups for all compared markers. The 5.8S rRNA gene offered only a limited phylogenetic resolution with low genetic divergence. ITS showed a high genetic divergence, but phylogenetic analyses were hampered by high variance in sequence lengths between clades.

Keywords: Chrysophyceae, SSU, LSU, COI, ITS

Received: November 8, 2016; Accepted: February 6, 2017; Prepublished online: August 23, 2017; Published: September 20, 2017  Show citation

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Bock, C., Chatzinotas, A., & Boenigk, J. (2017). Genetic diversity in chrysophytes: Comparison of different gene markers. Fottea, 17(2), 209-221. doi: 10.5507/fot.2017.005
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