Czech Phycology 4: 163-174, 2004

The Comet assay and the troubles with its application in the green alga Chlamydomonas reinhardtii

Kométový test a problémy s jeho aplikáciou u zelenej riasy Chlamydomonas reinhardtii

Barbara Sviežená1, Eliška Gálová1, Branislav Kusenda1, Miroslava Slaninová1, Daniel Vlček1, Mária Dušinská2
1 Comenius University, Faculty of Natural Sciences, Department of Genetics, Mlynská dolina B1, Bratislava, Slovakia, SK-842 15
2 Institute of Preventive and Clinical Medicine, Department of Molecular and Genetic Toxicology, Limbová 14, Bratislava, SK-801 03

The single cell gel electrophoresis or Comet assay is a sensitive, reliable, rapid and economic method for DNA double- and single-strand breaks, alkali-labile sites and delayed repair site detection, in eukaryotic individual cells. Given its overall characteristics, this method has been widely used over the past few years in several different areas. In this paper we review briefly the basic data about the principles, methodology and applications of this assay and we discuss also the possible explanation for not achieving comets to date in singlecell green alga Chlamydomonas reinhardtii.

Published: March 1, 2004  Show citation

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Sviežená, B., Gálová, E., Kusenda, B., Slaninová, M., Vlček, D., & Dušinská, M. (2004). The Comet assay and the troubles with its application in the green alga Chlamydomonas reinhardtii. Czech Phycology, 4(1), 163-174
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