Taxonomic review of the cyanoprokaryotic genera Planktothrix and Planktothricoides

Jiří Komárek, Jaroslava Komárková

Czech Phycology 4: 1-18, 2004  

The cyanoprokaryotic/cyanobacterial genera Planktothrix and Planktothricoides belong to the planktic filamentous phormidiacean types. They are well characterized and delimited by phenotype, ultrastructure, and mainly by genetic criteria (molecular sequencing). However, their intrageneric diversity, which is important for ecological research (eutrophication of water reservoirs, toxicity), is complicated, and the classification on the specific level is difficult. The problematics of species identification is discussed in the present review. - The study was supported by the grants EU "MIDI-CHIP EVK2-1999-00213", and GACR No. "KSK 6005114".

Review of cyanobacteria and algae of the Czech Republic

Aloisie Poulíčková, Oldřich Lhotský, Darina Dřímalová

Czech Phycology 4: 19-33, 2004  

Přehled řas Všebořického laloku (Česká republika) [in Czech]

Jana Ambrožová

Czech Phycology 4: 35-41, 2004  

Short term monitoring of numerous water reservoirs in the area of Všebořický lalok (district Ústí nad Labem, North-western Bohemia) was started as a partial study requested for considering the future use of the territory devastated by coal gas industry. Water chemistry of industrial water reservoirs has been strongly affected especially by coal mining in the surrounding area and by wastewaters rich in ammonium and phenol derivatives from industrial production. Coal gas production stopped in 1995. This study describes algal communities eight years after the termination of coal gas production. The study will serve to the town council of Ústí nad...

Desmids (Zygnematophyceae) of the spring fens of a part of West Carpathians

Miloslav Kitner, Aloisie Poulíčková, Radko Novotný, Michal Hájek

Czech Phycology 4: 43-61, 2004  

The occurrence, morphology and autecology of Desmidiales were studied in the Bílé Karpaty Mts. and Moravskoslezské Beskydy Mts., on the border between the Czech and Slovak Republic. A total of 40 taxa were found; species Actinotaenium cucurbitinum, Closterium cynthia var. latum, Cosmarium tetraophtalmum, Euastrum ansatum var. pyxidatum, Staurastrum senarium, Staurodesmus cuspidatus were recorded for the first time from the territory of the Czech Republic; species Closterium costatum, Cl. cynthia, Cl. lunula, Cosmarium cucumis, C. nasutum f. granulata, C. pachydermum, C. plicatum, Euastrum...

Pediastrum privum (PRINTZ) HEGEWALD in the Czech Republic

Rodan Geriš

Czech Phycology 4: 63-66, 2004  

Pediastrum privum is relatively rare coccal green algal species with circumpolar boreo-alpine distribution on the north hemisphere. It prefers clear nordic lakes and peat bogs. Its remnants can also occur in the paleobotanical findings. This inconspicuous alga can be easily overlooked and differs from other species of genus Pediastrum in its size and shape. In May 2002 I found this alga in a little pond near Staré Město pod Landštejnem close to the Austrian border. The pond catchment which is almost unaffected by human activities extend in a cold and wet climatic area of South Bohemia. Pediastrum privum has not been found in the...

A contribution to the knowledge of the silicascaled chrysophytes in eastern Hungary

Magda Řezáčová, Pavel Škaloud

Czech Phycology 4: 67-73, 2004  

Six species of the genus Mallomonas (Synurophyceae) were identified by electron microscopy (EM) from three localities in eastern Hungary. The composition of this flora was characteristic for warm, eutrophic and alkaline waters. Mallomonas acaroides, M. elongata, M. caudata and M. tonsurata are common and widely distributed. Mallomonas portae-ferreae is one of the typical members of Mallomonas species in summer phytoplankton in Hungary. Mallomonas peronoides var. bangladeshica was recorded in Europe for the second time.

Moss diatom (Bacillariophyceae) flora of the Nature Reserve Adršpašsko-Teplické Rocks (Czech Republic)

Jana Nováková, Aloisie Poulíčková

Czech Phycology 4: 75-86, 2004  

Epiphytic diatom flora on bryophytes of the genus Sphagnum was studied at 32 sites. Low species richness, altogether 15 diatom species, corresponds with very low pH at all sites. Eunotia paludosa, Eunotia exigua, Pinnularia rupestris and P. subcapitata were the most frequent species. Caloneis aerophila from the rock face is a new species for the Czech Republic. The abundance and vertical distribution of diatoms on bryophytes were influenced by the moisture of the site studied.

The variation of phytoplankton in different types of floodplain pools: a case study from the River Morava floodplain
(the Czech Republic)

Alena Kočárková, Martin Duchoslav, Aloisie Poulíčková

Czech Phycology 4: 87-102, 2004  

The composition and dynamics of phytoplankton communities in different types of floodplain pools (the River Morava floodplain, the Czech Republic) were studied in relation to selected physical and chemical parameters. Significant differences in phytoplankton were found between meadow and forest types of pools. On the contrary, no significant differences were found between periodical and permanent types of pools and between natural and artificial pools. Seasonal dynamics of phytoplankton is significant and is influenced by temperature course during the year.

The influence of Hydrodictyon reticulatum (L.) LAGERH. on diurnal changes in environmental variables in a shallow pool

Eva Lelková, Aloisie Poulíčková

Czech Phycology 4: 103-109, 2004  

The presented experiment aimed to evaluate the influence of Hydrodictyon reticulatum on diurnal changes in environmental variables of water. Regular measurements of temperature, oxygen concentration and pH were carried out during 26 hours. Remarkable diurnal changes of oxygen concentration and pH values were observed in vessels containing H. reticulatum.

Phytoplankton ecology of two floodplain pools near Olomouc

Eva Lelková, Alena Kočárková, Aloisie Poulíčková

Czech Phycology 4: 111-121, 2004  

A three-year study of selected environmental variables, species composition and phytoplankton abundance was carried out in two floodplain pools. Altogether, 123 taxa of algae and cyanobacteria were identified. Euglenophyta, Cryptophyta and Bacillariophyceae were diverse and dominant groups, especially species Cryptomonas curvata, C. marssonii, C. reflexa, Euglena hemichromata, Trachelomonas hispida and T. volvocina occurred in samples regularly. Mass occurrence of Hydrodictyon reticulatum was observed in one of the pools.

Algae and cyanobacteria in freshwater sponges (Porifera) in the Dalešice and Mohelno Reservoirs [in Czech]

Zdeňka Žáková, Petr Marvan

Czech Phycology 4: 123-132, 2004  

In 2001-2002, the evaluation of the occurrence of algae and cyanobacteria in freshwater sponges (Porifera) was performed in the Dalešice - Mohelno System of Reservoirs within the research project "Role and seasonal changes of freshwater sponges (Porifera) in the reservoir influenced by the nuclear power plant operation" (Grant agency CR No. 206/01/1595). We found 130 species of algae and cyanobacteria in Porifera growing in the depth of 6-10 m under water surface in Dalešice Reservoir (10.2 km below the mouth of the Jihlava River) and in Mohelno Reservoir 0.2 km from the dam. In spite of the fact that the phytoplankton growth in Dalešice Reservoir...

The survival and vitality of cyanobacteria and algae in fishpond bottom sediments

Petr Hašler, Aloisie Poulíčková, Monika Lysáková

Czech Phycology 4: 133-144, 2004  

The extraction of sediment cores took place in January 2003 during total ice covering of the investigated fishpond. Sediment cores were cut into 3-cm-thick layers. Sediment samples were cultured and vitality of cyanobateria and algae was studied. Altogether, we identified 49 species of cyanobacteria and algae. Green algae were the most frequent and vital group. Fishpond phytoplankton was dominated by Planktothrix agardhii (GOMONT) ANAGNOSTIDIS et KOMÁREK during last 5 years. Cyanobacteria occurred sporadically in sediments.

Ecological study of phytoplankton of the Anzali lagoon (N Iran) and its outflow into the Caspian Sea

Zohreh Ramezanpoor

Czech Phycology 4: 145-154, 2004  

A study of phytoplankton communities of western regions of the Anzali lagoon, rivers flowing from the lagoon, and Anzali Harbor (navigation channel), located in the southern coast of the Caspian Sea was carried out. Samples were collected monthly at nine sites from May 1992 to April 1993 from the surface, 1 m, 5 m and from the final depths of each sampling site using Ruttner sampler with 1 lit. volume. Water and air temperature, turbidity, pH, Cl^-, PO₄^3-, NO^3-, SO₄^2- and salinity were measured. Phytoplankton samples were fixed in 4% formaldehyde solution and then identified and counted...

Some finds of subaerophytic cyanobacteria on wetted walls of La Palma (Canary Islands)

Pavel Hrouzek, Jaroslav Šoun

Czech Phycology 4: 155-162, 2004  

Randomly selected samples of cyanobacterial mats growing on wetted walls of two distinct localities (Barranco de las Augustias, and Los Tilos) in La Palma (the Canary Islands) were collected. The collected samples contained twelve cyanobacteria morphotypes (determined according traditional system). Populations of both localities were dominated by coccoid cyanobacteria (Aphanothece pallida and Aphanothece cf. saxicola) and in some sampling sites by filamentous cyanobacteria Nostoc spp. and Leptolyngbya spp. In spite of the fact that the Canary Islands are characterized by a high level of species endemism mainly in terrestrial plants, most of the isolated...

The Comet assay and the troubles with its application in the green alga Chlamydomonas reinhardtii

Barbara Sviežená, Eliška Gálová, Branislav Kusenda, Miroslava Slaninová, Daniel Vlček, Mária Dušinská

Czech Phycology 4: 163-174, 2004  

The single cell gel electrophoresis or Comet assay is a sensitive, reliable, rapid and economic method for DNA double- and single-strand breaks, alkali-labile sites and delayed repair site detection, in eukaryotic individual cells. Given its overall characteristics, this method has been widely used over the past few years in several different areas. In this paper we review briefly the basic data about the principles, methodology and applications of this assay and we discuss also the possible explanation for not achieving comets to date in singlecell green alga Chlamydomonas reinhardtii.

Water blooms in recreational waters of Slovak Republic [in Slovak] [in Czech]

Mária Horecká, Viera Nagyová, Jana Košťálová

Czech Phycology 4: 175-182, 2004  

The recreational water quality in Slovakia has been monitored including the observation of water blooms occurrence, testing of their toxicity and the determination of microcystin content by HPLC method. At the same time the free water was tested on toxicity with Thamnocephalus platyurus, Sinapis alba, Poecilia reticulata and on the content of microcystins. The toxic water bloom was observed on 12 or 14 lokalities annualy. The toxicity of water was confirmed in case of massive water blooms development in reservoirs Zemplínska Šírava, Liptovská Mara, Kanianka, Kuchajda and the others. The limit 100 000 cyanobacteria cells in 1 ml was discused...

Confocal microscopy of the green-algal chloroplast

Pavel Škaloud, Barbora Radochová

Czech Phycology 4: 183-190, 2004  

The confocal microscopy proves useful in the investigations of chloroplast morphology and ontogeny of several coccal green algae. The three-dimensional images allow the reconstruction of the chloroplast morphology and the exact determination of the chloroplast number in the cell. This character is essential for species determination in numerous genera of coccal green algae. The simple method of pyrenoid visualisation by confocal images is demonstrated. Within the pyrenoid we can recognise the structure and shape of the invaginated thylakoids. In comparison with the methodically difficult transmission electron microscopy, the confocal microscopy provides...

Vliv mineralizace vzorku na citlivost řasového biotestu [in Czech]

Vladimír Kočí, Hynek Beneš, Petr Hašler

Czech Phycology 4: 191-201, 2004  

The effect of variable salinity on toxicity response of planktonic green alga- Scenedesmus subspicatus to heavy metals (especially on chrom VI) exposition was determined. Scenedesmus subspicatus was cultivated in suspension culture based on ISO guideline and salinity gradient was arranged by anorganic salt mixture. Salt mixture was based on composition of marine water. The algae were grown at salinity 0.006 mol.l^-1, 0.031 mol.l^-1, 0.056 mol.l^-1, 0.082 mol.l^-1 and 0.107 mol.l^-1. At all chosen salinity levels a dose-response curves of S. subspicatus exposed to K2Cr2O7 were determined. The results...